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热带作物学报 ›› 2020, Vol. 41 ›› Issue (5): 929-938.doi: 10.3969/j.issn.1000-2561.2020.05.012

• 生物技术与组织培养 • 上一篇    下一篇

基于SRAP标记的国兰种质资源遗传多样性分析

袁媛1,曹彬1,张咏琪1,陈清西1,*(),陈南川2   

  1. 1. 福建农林大学园艺学院,福建福州 350002
    2. 福建百秾生态科技有限公司,福建诏安 363500
  • 收稿日期:2019-08-05 修回日期:2019-08-26 出版日期:2020-05-25 发布日期:2020-06-15
  • 通讯作者: 陈清西 E-mail:cqx0246@fafu.edu.cn
  • 作者简介:袁 媛(1988—),女,博士,讲师,研究方向:花卉生理与分子生物学。
  • 基金资助:
    福建农林大学科技创新专项基金项目(CXZX2018087)

Genetic Diversity Analysis of Cymbidium Germplasms Based on SRAP Markers

YUAN Yuan1,CAO Bin1,ZHANG Yongqi1,CHEN Qingxi1,*(),CHEN Nanchuan2   

  1. 1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2. Fujian Bainong Ecological Technology Co., Ltd, Zhao’an, Fujian 363500, China
  • Received:2019-08-05 Revised:2019-08-26 Online:2020-05-25 Published:2020-06-15
  • Contact: CHEN Qingxi E-mail:cqx0246@fafu.edu.cn

摘要:

采用SRAP(sequence related amplified polymorphism)技术对154份国兰(杂交兰)种质资源的亲缘关系进行分析。从168对SRAP引物中筛选出16对条带多、带型清晰、多态性强的引物组合对所有样品进行扩增,共获得874条带,其中多态性条带857条,多态性比率为98.1%,平均每对引物每个样品产生5.74条带。UPGMA聚类分析表明:Me6-Em3引物能较好地揭示154份国兰种质间的遗传多样性与亲缘关系,在遗传相似系数0.818处将它们分为8个类群,遗传相似系数变化范围为0.772~1.000。此外,发现引物Me8-Em4、Me11-Em2和Me12-Em11可以较可靠地联合鉴定建兰。该研究结果可为国兰种质资源利用及杂种后代鉴定提供参考。

关键词: 国兰, 种质资源, SRAP标记, 遗传多样性

Abstract:

SRAP (sequence related amplified polymorphism) was applied for the determination of the diversity of 154 samples of Cymbidium germplasm. 16 SRAP primer pairs were screened from 168 SRAP primers and applied to all the materials, resulting in 874 amplified DNA bands. For the amplified DNA bands, 857 were polymorphic, with a ratio of 98.1%. The average number of bands of each SRAP primer for each Cymbidium germplasm was 5.74. UPGMA cluster analysis showed that the genetic diversity and genetic relationship of the Cymbidium germplasms could be revealed by Me6-Em3 primer pair and could be classified into eight groups with a similarity coefficient 0.818 and the genetic similarity of the germplasms ranged from 0.772 to 1.000. It was found for the first time that the primer pairs Me8-Em4, Me11-Em2 and Me12-Em11 could be used together to identify C. Ensifolium reliably. Results in the present study could provide references for the utilization of Cymbidium germplasm and the identification of Cymbidium hybrid cultivars.

Key words: Chinese Cymbidium, germplasm, SRAP marker, genetic diversity

中图分类号: 

  • S682.31